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B-hPTH mice
Strain Name
C57BL/6N-Pthtm1(PTH)Bcgen/Bcgen
Common Name  B-hPTH mice
Background C57BL/6N Catalog number 112633
Aliases 
FIH11

Protein expression analysis

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Strain specific PTH expression analysis in homozygous B-hPTH mice by ELISA. Serum were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hPTH mice (H/H). Human PTH was exclusively detectable in homozygous B-hPTH mice.


mRNA expression analysis


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Strain specific analysis of PTH mRNA expression in wild-type C57BL/6 mice and B-hPTH mice by RT-PCR. Thymus RNA was isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hPTH mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human PTH primers. Mouse Pth mRNA was detectable only in wild-type C57BL/6 mice. Human PTH mRNA was detectable only in homozygous B-hPTH mice but not in wild-type mice.