B-hSAA1 mice_Biocytogen Pharmaceuticals (Beijing) Co., Ltd._Biocytogen, drug development, antibody discovery

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동물/세포 모델

동물/세포모델

Humanized Mouse Models

Other Humanized Mice

Text

B-hSAA1 mice

Strain Name	C57BL/6N-Saa^{tm1(SAA)Bcgen}/Bcgen	Common Name	B-hSAA1 mice
Background	C57BL/6N	Catalog number	113067
Related Genes	SAA1; SAA; PIG4; SAA2; TP53I4
NCBI Gene ID	6288

Description

Serum amyloid A proteins (SAAs) are a family of homologous apolipoproteins of high density lipoprotein (HDL). They can be divided into two groups. The first group consists of the acute phase SAA1 and SAA2 that associate with HDL during inflammation and remodel the HDL particle by displacing apolipoprotein A1. The second group consists of constitutively expressed SAA4 and SAA5 that exist as minor apolipoproteins on HDL but make up more than 90% of the total SAA during homeostasis.

Protein expression analysis in serum

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Strain specific SAA expression analysis in wild-type C57BL/6 mice and homozygous humanized B-hSAA1 mice by ELISA. Serum was collected from female wild-type C57BL/6 mice (+/+) (n=3, 6-week-old) and homozygous B-hSAA1 mice (H/H) (n=3, 6-week-old). Expression level of mouse and human SAA1 were analyzed by ELISA (Human SAA ELISA Kit (ab100635), abcam, ab100635; Mouse SAA ELISA Kit, R&D, MSAA00). Mouse SAA was only detectable in wild-type C57BL/6 mice. Human SAA was exclusively detectable in homozygous B-hSAA1 mice (n=3). Values are expressed as mean ± SEM.

mRNA expression analysis

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Strain specific analysis of SAA1 mRNA expression in wild-type C57BL/6 mice and B-hSAA1 mice by RT-PCR. Liver RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hSAA1 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human SAA1 primers. Mouse Saa1 mRNA was detectable only in wild-type C57BL/6 mice. Human SAA1 mRNA was detectable only in homozygous B-hSAA1 mice but not in wild-type mice.