Strain Name
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C57BL/6N-Serpinf2tm1(SERPINF2)Bcgen/Bcgen
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Common Name
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B-hSERPINF2 mice
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Background
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C57BL/6N
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Catalog number
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112340
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Aliases
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A2AP, AAP, ALPHA-2-PI, API, PLI, alpha2AP
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Protein expression analysis
Strain specific SERPINF2 expression analysis in homozygous B-hSERPINF2 mice by ELISA. Plasma was isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hSERPINF2 mice (H/H) and analyzed by ELISA with species-specific SERPINF2 ELISA kit. Human SERPINF2 was exclusively detectable in homozygous B-hSERPINF2 mice but not in wild-type mice.
mRNA expression analysis
Strain specific analysis of SERPINF2 mRNA expression in wild-type C57BL/6 mice and B-hSERPINF2 mice by RT-PCR. Liver RNA was isolated from wildtype C57BL/6 mice (+/+) and homozygous B-hSERPINF2 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human SERPINF2 primers. Mouse Serpinf2 mRNA was detectable in wild-type C57BL/6 mice. Human SERPINF2 mRNA was detectable only in homozygous B-hSERPINF2 mice but not in wild-type mice.