Strain Name
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C57BL/6-Shhtm1(SHH)Bcgen/Bcgen
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Common Name
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B-hSHH mice
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Background
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C57BL/6
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Catalog number
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111903
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Aliases
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SHH: TPT; HHG1; HLP3; HPE3; SMMCI; ShhNC; TPTPS; MCOPCB5
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Protein expression analysis
Western blot analysis of SHH protein expression in homozygous B-hSHH mice. Various tissue lysates were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hSHH mice (H/H) , and then analyzed by western blot with species-specific anti-SHH antibody. 40 μg total proteins were loaded for western blotting analysis. SHH was detected in stomach, liver and kidney in both wild-type and homozygous B-hSHH mice, as anti-SHH antibody was cross-reactive between human and mouse. SHH was low expression in lung.
mRNA expression analysis
Strain specific analysis of SHH mRNA expression in wild-type C57BL/6 mice and B-hSHH mice by RT-PCR. Stomach RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hSHH mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human SHH primers. Mouse Shh mRNA was detectable only in wild-type C57BL/6 mice. Human SHH mRNA was detectable only in homozygous B-hSHH mice but not in wild-type mice.