B-hSIGLEC1 mice_Biocytogen Pharmaceuticals (Beijing) Co., Ltd._Biocytogen, drug development, antibody discovery

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동물/세포 모델

동물/세포모델

Humanized Mouse Models

Other Humanized Mice

Text

B-hSIGLEC1 mice

Strain Name	C57BL/6-Siglec1^{tm2(SIGLEC1)Bcgen}/Bcgen	Common Name	B-hSIGLEC1 mice
Background	C57BL/6	Catalog number	112471
Related Genes	CD169, SIGLEC-1, SN
NCBI Gene ID	6614

Description

Siglec-1, also known as CD169 and Sialoadhesin (Sn), is a type I lectin containing 17 immunoglobulin (Ig) domains (one variable domain and 16 constant domains), which involved in macrophage binding to different cell types such as granulocytes, monocytes, NK, B, and T cells.
The exons 2-19 of mouse Siglec1 gene that encode extracellular domain and part of transmembrane domain are replaced by human counterparts in B-hSIGLEC1 mice. The genomic region of mouse Siglec1 gene that encodes signal peptide and cytoplasmic portion is retained. The promoter, 5’UTR and 3’UTR region of the mouse gene are also retained. The chimeric SIGLEC1 expression is driven by endogenous mouse Siglec1 promoter, while mouse Siglec1 gene transcription and translation will be disrupted.
Mouse SIGLEC1 was detectable on NK cells, monocytes, macrophages and DCs and from spleen of wild-type C57BL/6JNifdc mice.
Mouse SIGLEC1 was detectable on macrophages from blood of wild-type C57BL/6JNifdc mice.
Mouse SIGLEC1 was detectable on T cells, B cells and NK cells from lymph nodes of wild-type C57BL/6JNifdc mice.
Human SIGLEC1 was detectable on NK cells，monocytes, macrophages and DCs and from spleen of B-hSIGLEC1 mice.
Human SIGLEC1 was detectable on macrophages from blood of B-hSIGLEC1 mice.
Human SIGLEC1 was detectable on T cells, B cells and NK cells from lymph nodes of B-hSIGLEC1 mice.
Application: This product is used for pharmacodynamics and safety evaluation of autoimmunity drugs.

Protein expression analysis-spleen

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Strain specific SIGLEC1 expression analysis in homozygous B-hSIGLEC1 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6JNifdc mice (+/+) and homozygous B-hSIGLEC1 mice (H/H), and protein expression was analyzed with anti-mouse SIGLEC1 antibody (Biolegend, 142403) and anti-human SIGLEC1 antibody (Biolegend, 346007) by flow cytometry. mSIGLEC1 was detectable in NK cells of wild-type C57BL/6JNifdc mice, but not T cells and B cells. hSIGLEC1 was detectable in NK cells of homozygous B-hSIGLEC1 mice, but not T cells and B cells.

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Strain specific SIGLEC1 expression analysis in homozygous B-hSIGLEC1 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6JNifdc mice (+/+) and homozygous B-hSIGLEC1 mice (H/H), and protein expression was analyzed with anti-mouse SIGLEC1 antibody (Biolegend, 142403) and anti-human SIGLEC1 antibody (Biolegend, 346007) by flow cytometry. mSIGLEC1 was detectable in monocytes and macrophages of wild-type C57BL/6JNifdc mice. hSIGLEC1 was detectable in monocytes and macrophages of homozygous B-hSIGLEC1 mice.

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Strain specific SIGLEC1 expression analysis in homozygous B-hSIGLEC1 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6JNifdc mice (+/+) and homozygous B-hSIGLEC1 mice (H/H), and protein expression was analyzed with anti-mouse SIGLEC1 antibody (Biolegend, 142403) and anti-human SIGLEC1 antibody (Biolegend, 346007) by flow cytometry. mSIGLEC1 was detectable in DCs of wild-type C57BL/6JNifdc mice, but not neutrophils. hSIGLEC1 was detectable in DCs of homozygous B-hSIGLEC1 mice, but not neutrophils.

Protein expression analysis-blood

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Strain specific SIGLEC1 expression analysis in homozygous B-hSIGLEC1 mice by flow cytometry. Blood cells were collected from wild-type C57BL/6JNifdc mice (+/+) and homozygous B-hSIGLEC1 mice (H/H), and protein expression was analyzed with anti-mouse SIGLEC1 antibody (Biolegend, 142403) and anti-human SIGLEC1 antibody (Biolegend, 346007) by flow cytometry. mSIGLEC1 was not detectable in T cells, B cells and NK cells of wild-type C57BL/6JNifdc mice. hSIGLEC1 was not detectable in T cells, B cells and NK cells of homozygous B-hSIGLEC1 mice.

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Strain specific SIGLEC1 expression analysis in homozygous B-hSIGLEC1 mice by flow cytometry. Blood cells were collected from wild-type C57BL/6JNifdc mice (+/+) and homozygous B-hSIGLEC1 mice (H/H), and protein expression was analyzed with anti-mouse SIGLEC1 antibody (Biolegend, 142403) and anti-human SIGLEC1 antibody (Biolegend, 346007) by flow cytometry. mSIGLEC1 was detectable in macrophages of wild-type C57BL/6JNifdc mice, but not monocytes. hSIGLEC1 was detectable in macrophages of homozygous B-hSIGLEC1 mice, but not monocytes.

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Strain specific SIGLEC1 expression analysis in homozygous B-hSIGLEC1 mice by flow cytometry. Blood cells were collected from wild-type C57BL/6JNifdc mice (+/+) and homozygous B-hSIGLEC1 mice (H/H), and protein expression was analyzed with anti-mouse SIGLEC1 antibody (Biolegend, 142403) and anti-human SIGLEC1 antibody (Biolegend, 346007) by flow cytometry. mSIGLEC1 was not detectable in DCs and neutrophils of wild-type C57BL/6JNifdc mice. hSIGLEC1 was not detectable in DCs and neutrophils of homozygous B-hSIGLEC1 mice.

Protein expression analysis-lymph nodes

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Strain specific SIGLEC1 expression analysis in homozygous B-hSIGLEC1 mice by flow cytometry. Lymph nodes were collected from wild-type C57BL/6JNifdc mice (+/+) and homozygous B-hSIGLEC1 mice (H/H), and protein expression was analyzed with anti-mouse SIGLEC1 antibody (Biolegend, 142403) and anti-human SIGLEC1 antibody (Biolegend, 346007) by flow cytometry. mSIGLEC1 was detectable in T cells, B cells and NK cells of wild-type C57BL/6JNifdc mice. hSIGLEC1 was detectable in T cells, B cells and NK cells of homozygous B-hSIGLEC1 mice.