top
Please input keywords
Order
*Country
한국
미국
일본
중국
영국
호주
프랑스
독일
이탈리아
싱가포르
인도
러시아
캐나다
스위스
기타 국가
*Province
*City
*Name
*Telephone
*Company
*Position
*Email
*Verification code
*Verification Code
B-hTCIRG1 mice
Strain Name
C57BL/6-Tcirg1tm1(TCIRG1)Bcgen/Bcgen
Common Name  B-hTCIRG1 mice
Background C57BL/6 Catalog number 112214
Aliases 

TCIRG1: a3, Stv1, Vph1, Atp6i, OC116, OPTB1, TIRC7, ATP6N1C, ATP6V0A3, OC-116kDa

mRNA expression analysis

from clipboard


Strain specific analysis of TCIRG1 mRNA expression in wild-type C57BL/6 mice and B-hTCIRG1 mice by RT-PCR. Spleen RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hTCIRG1 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human TCIRG1 primers. Mouse Tcirg1 mRNA was detectable only in wild-type C57BL/6 mice. Human TCIRG1 mRNA was detectable only in homozygous B-hTCIRG1 mice but not in wild-type mice. 

Protein expression analysis

from clipboard


Western blot analysis of TCIRG1 protein expression in homozygous B-hTCIRG1 mice. Various tissue lysates were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hTCIRG1 mice (H/H), and then analyzed by western blot with cross-reactive anti-TCIRG1 antibody. 20 μg total proteins were loaded for western blotting analysis. TCIRG1 was detected in spleen, lymph node, lung, adrenal gland and bone marrow.