Strain specific analysis of TEM7R mRNA expression in wild-type C57BL/6 mice and B-hTEM7R mice by RT-PCR. Lung and kidney RNA were isolated from wildtype C57BL/6 mice (+/+) and homozygous B-hTEM7R mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human TEM7R primers. Mouse Tem7r mRNA was only detectable in wild-type C57BL/6 mice. Human TEM7R mRNA was only detectable in homozygous B-hTEM7R mice but not in wild-type mice. 

Protein expression analysis

Western blot analysis of TEM7R protein expression in homozygous B-hTEM7R mice. Various tissue lysates were collected from wild-type C57BL/6N (+/+) mice and homozygous B-hTEM7R mice (H/H), and then analyzed by western blot with anti-TEM7R antibody. 30 μg total proteins were loaded for western blotting analysis. TEM7R was detected in kidney, bladder and cerebellum.