Strain specific TGFA expression analysis in wild-type C57BL/6 mice and homozygous humanized B-hTGFA mice by ELISA. Serum was collected from wild-type C57BL/6 mice (+/+) and homozygous B-hTGFA mice (H/H) (n=3, 10-week-old). Protein expression level of TGFA was analyzed by ELISA. Human TGFA was exclusively detectable in homozygous B-hTGFA mice

Strain specific TGFA expression analysis in wild-type C57BL/6 mice and homozygous humanized B-hTGFA mice by ELISA. BMDM was collected from wild-type C57BL/6 mice (+/+) and homozygous B-hTGFA mice (H/H), stimulated with 10 μg/mL ConA for 5d. Protein expression level of TGFA from cell culture supernatant was analyzed by ELISA. Human TGFA was exclusively detectable in homozygous B-hTGFA mice.

Strain specific analysis of TGFA mRNA expression in wild-type C57BL/6 mice and B-hTGFA mice by RT-PCR. Lung RNA were isolated from wildtype C57BL/6 mice (+/+) and homozygous B-hTGFA mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human TGFA primers. Mouse Tgfa mRNA was detectable only in wild-type C57BL/6 mice. Human TGFA mRNA was detectable only in homozygous B-hTGFA mice but not in wild-type mice.