• Expression: TM4SF1 is a protein with four transmembrane domains, which is highly expressed on lung, breast, colon, and ovarian carcinomas. It is also present on normal endothelial cells.  
• Application: This mice model can be used for pharmacodynamics and safety evaluation of colon, breast and other cancers.

Strain specific analysis of TM4SF1 mRNA expression in wild-type C57BL/6 mice and B-hTM4SF1 mice by RT-PCR. Lung RNA was isolated from wildtype C57BL/6 mice (+/+) and homozygous B-hTM4SF1 mice (H/H), then cDNA libraries was synthesized by reverse transcription, followed by PCR with mouse or human TM4SF1 primers. Mouse Tm4sf1 mRNA was only detectable in wild-type mice. Human TM4SF1 mRNA was exclusively detectable in homozygous B-hTM4SF1 mice but not in wild-type mice. 

Strain specific TM4SF1 expression analysis in homozygous B-hTM4SF1 mice by flow cytometry. Lung endothelial cells were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hTM4SF1 mice (H/H), and analyzed by flow cytometry with anti-humanTM4SF1 antibody (R&D, FAB8164R). hTM4SF1 was detectable on lung endothelial cells of homozygous mice.