Western blot analysis of TSPAN8 protein expression in homozygous B-hTSPAN8 mice. Various tissue lysates were collected from wild-type C57BL/6 mice (+/+)  and homozygous B-hTSPAN8 mice (H/H), and then analyzed by western blot with anti-TSPAN8 antibody (abcam, ab70007). 40 μg total proteins were loaded for western blotting analysis. hTSPAN8 was detected in stomach, small intestine, colon, kidney and brain. HCT116 cell is positive control. KO tissues are negative control.

Strain specific analysis of TSPAN8 mRNA expression in wild-type C57BL/6 mice and B-hTSPAN8 mice by RT-PCR. Small intestine RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hTSPAN8 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human TSPAN8 primers. Mouse Tspan8 mRNA were detectable only in wild-type C57BL/6 mice. Human TSPAN8 mRNA was detectable only in homozygous B-hTSPAN8 mice but not in wild-type mice.