Strain Name
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C57BL/6-Ttrtm1(TTR*V30M)Bcgen/Bcgen
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Common Name
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B-hTTR*V30M mice
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Background
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C57BL/6
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Catalog number
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112386
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Aliases
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CTS, TTN, ATTR, CTS1, PALB, TBPA, HEL111, HsT2651
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mRNA expression analysis
Strain specific analysis of TTR*V30M mRNA expression in wild-type C57BL/6 mice and B-hTTR*V30M mice by RT-PCR. Liver RNA was isolated from wildtype C57BL/6 mice (+/+) and heterozygous B-hTTR*V30M mice (H/+), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human TTR primers. Mouse Ttr mRNA was detectable in wild-type C57BL/6 mice and heterozygous B-hTTR*V30M mice. Human TTR mRNA was detectable only in heterozygous B-hTTR*V30M mice but not in wild-type mice.
Protein expression analysis
Strain specific TTR expression analysis in homozygous B-hTTR*V30M mice by ELISA. Plasma were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hTTR*V30M mice (H/H) and analyzed by ELISA with species-specific TTR ELISA kit. Mouse TTR was detectable in wild-type mice (A). Human TTR was exclusively detectable in homozygous B-hTTR*V30M mice (B).