Strain Name
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C57BL/6N-Vldlrtm1(VLDLR)Bcgen/Bcgen
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Common Name
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B-hVLDLR mice
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Background
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C57BL/6N
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Catalog number
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111195
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Aliases
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VLDLR: CAMRQ1; CARMQ1; CHRMQ1; VLDL-R; VLDLRCH
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Protein expression analysis
Strain specific VLDLR expression analysis in homozygous B-hVLDLR mice by western blot. Various tissue lysates were collected from wild-type C57BL/6N (+/+) mice and homozygous B-hVLDLR mice (H/H), and then analyzed by western blot with anti-VLDLR antibody. 40μg total proteins were loaded for western blotting analysis. VLDLR was detected in heart, lung, brain and testis.
mRNA expression analysis
Strain specific analysis of VLDLR mRNA expression in wild-type C57BL/6N mice and B-hVLDLR mice by RT-PCR. Testis and brain RNA were isolated from wild-type C57BL/6N mice (+/+) and homozygous B-hVLDLR mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human VLDLR primers. Mouse Vldlr mRNA was detectable only in wild-type C57BL/6N mice. Human VLDLR mRNA was detectable only in homozygous B-hVLDLR mice but not in wild-type mice.