B-hCD16A/hNKP46 mice(CB-17 SCID)_Biocytogen Pharmaceuticals (Beijing) Co., Ltd._Biocytogen, drug development, antibody discovery

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동물/세포 모델

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B-hCD16A/hNKP46 mice(CB-17 SCID)

Strain Name	CB17/lcr-Prkdc^scidFcgr4^{tm2(FCGR3A)Bcge}ⁿ Ncr1^{tm1(NCR1)Bcgen}/Bcgen	Common Name	B-hCD16A/hNKP46 mice(CB-17 SCID)
Background	CB-17 SCID	Catalog number	112987
Related Genes	CD16, FCG3, CD16A, FCGR3, IGFR3, IMD20, FCR-10, FCRIII, FCGRIII, FCRIIIA, LY94, CD335, NKP46, NK-p46
NCBI Gene ID	246256, 17086

Protein expression analysis in spleen-NKP46

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Strain specific NKP46 expression analysis in wild-type CB-17 SCID mice and homozygous B-hCD16A/hNKP46 mice(CB-17 SCID) by flow cytometry. Splenocytes were collected from wild-type CB-17 SCID mice (+/+) and homozygous B-hCD16A/hNKP46 mice(CB-17 SCID) (H/H) (n=3, 6-week old). Protein expression was analyzed with anti-mouse NKP46 antibody (eBioscience™, 17-3351-82) and anti-human NKP46 antibody (Biolegend, 331916) by flow cytometry. Mouse NKP46 was only detectable in wild-type CB-17 SCID mice. Human NKP46 was exclusively detectable in homozygous B-hCD16A/hNKP46 mice(CB-17 SCID), but not in wild-type CB-17 SCID mice.

Protein expression analysis in blood-NKP46

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Strain specific NKP46 expression analysis in wild-type CB-17 SCID mice and homozygous B-hCD16A/hNKP46 mice(CB-17 SCID) by flow cytometry. Blood were collected from wild-type CB-17 SCID mice (+/+) and homozygous B-hCD16A/hNKP46 mice(CB-17 SCID) (H/H) (n=3, 6-week old). Protein expression was analyzed with anti-mouse NKP46 antibody (eBioscience™, 17-3351-82) and anti-human NKP46 antibody (Biolegend, 331916) by flow cytometry. Mouse NKP46 was only detectable in wild-type CB-17 SCID mice. Human NKP46 was exclusively detectable in homozygous B-hCD16A/hNKP46 mice(CB-17 SCID), but not in wild-type CB-17 SCID mice.

Protein expression analysis in bone marrow-NKP46

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Strain specific NKP46 expression analysis in wild-type CB-17 SCID mice and homozygous B-hCD16A/hNKP46 mice(CB-17 SCID) by flow cytometry. Bone marrow were collected from wild-type CB-17 SCID mice (+/+) and homozygous B-hCD16A/hNKP46 mice(CB-17 SCID) (H/H) (n=3, 6-week old). Protein expression was analyzed with anti-mouse NKP46 antibody (eBioscience™, 17-3351-82) and anti-human NKP46 antibody (Biolegend, 331916) by flow cytometry. Mouse NKP46 was only detectable in wild-type CB-17 SCID mice. Human NKP46 was exclusively detectable in homozygous B-hCD16A/hNKP46 mice(CB-17 SCID), but not in wild-type CB-17 SCID mice.

Protein expression analysis in spleen NK cells-CD16A

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Strain specific CD16A expression analysis in wild-type CB-17 SCID mice and homozygous B-hCD16A/hNKP46 mice(CB-17 SCID) by flow cytometry. Splenocytes were collected from wild-type CB-17 SCID mice (+/+) and homozygous B-hCD16A/hNKP46 mice(CB-17 SCID) (H/H) (n=3, 6-week old). Protein expression was analyzed with anti-mouse CD16.2 (FcγRIV) antibody (Biolegend, 149503) and anti-human CD16 antibody (Biolegend, 302008) by flow cytometry. Mouse CD16A was only detectable in wild-type CB-17 SCID mice. Human CD16A was exclusively detectable in homozygous B-hCD16A/hNKP46 mice(CB-17 SCID), but not in wild-type CB-17 SCID mice.

Protein expression analysis in spleen DC cells-CD16A

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Protein expression analysis in spleen monocytes-CD16A

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Protein expression analysis in spleen neutrophils-CD16A

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Strain specific CD16A expression analysis in wild-type CB-17 SCID mice and homozygous B-hCD16A/hNKP46 mice(CB-17 SCID) by flow cytometry. Splenocytes were collected from wild-type CB-17 SCID mice (+/+) and homozygous B-hCD16A/hNKP46 mice(CB-17 SCID) (H/H) (n=3, 6-week old). Protein expression was analyzed with anti-mouse CD16.2 (FcγRIV) antibody (Biolegend, 149503) and anti-human CD16 antibody (Biolegend, 302008) by flow cytometry. Mouse CD16A was only detectable in wild-type CB-17 SCID mice. Human CD16A was not detectable in homozygous B-hCD16A/hNKP46 mice(CB-17 SCID).

Protein expression analysis in blood NK cells-CD16A

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Strain specific CD16A expression analysis in wild-type CB-17 SCID mice and homozygous B-hCD16A/hNKP46 mice(CB-17 SCID) by flow cytometry. Blood were collected from wild-type CB-17 SCID mice (+/+) and homozygous B-hCD16A/hNKP46 mice(CB-17 SCID) (H/H) (n=3, 6-week old). Protein expression was analyzed with anti-mouse CD16.2 (FcγRIV) antibody (Biolegend, 149503) and anti-human CD16 antibody (Biolegend, 302008) by flow cytometry. Mouse CD16A was detectable in wild-type CB-17 SCID mice. Human CD16A was exclusively detectable in homozygous B-hCD16A/hNKP46 mice(CB-17 SCID), but not in wild-type CB-17 SCID mice.

Protein expression analysis in blood DC cells-CD16A

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Protein expression analysis in blood monocytes-CD16A

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Strain specific CD16A expression analysis in wild-type CB-17 SCID mice and homozygous B-hCD16A/hNKP46 mice(CB-17 SCID) by flow cytometry. Blood were collected from wild-type CB-17 SCID mice (+/+) and homozygous B-hCD16A/hNKP46 mice(CB-17 SCID) (H/H) (n=3, 6-week old). Protein expression was analyzed with anti-mouse CD16.2 (FcγRIV) antibody (Biolegend, 149503) and anti-human CD16 antibody (Biolegend, 302008) by flow cytometry. Mouse CD16A was weakly detectable in wild-type CB-17 SCID mice. Human CD16A was exclusively detectable in homozygous B-hCD16A/hNKP46 mice(CB-17 SCID), but not in wild-type CB-17 SCID mice.

Protein expression analysis in blood macrophages-CD16A

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Strain specific CD16A expression analysis in wild-type CB-17 SCID mice and homozygous B-hCD16A/hNKP46 mice(CB-17 SCID) by flow cytometry. Blood were collected from wild-type CB-17 SCID mice (+/+) and homozygous B-hCD16A/hNKP46 mice(CB-17 SCID) (H/H) (n=3, 6-week old). Protein expression was analyzed with anti-mouse CD16.2 (FcγRIV) antibody (Biolegend, 149503) and anti-human CD16 antibody (Biolegend, 302008) by flow cytometry. Mouse CD16A was not detectable in wild-type CB-17 SCID mice. Human CD16A was weekly detectable in homozygous B-hCD16A/hNKP46 mice(CB-17 SCID), but not in wild-type CB-17 SCID mice.

Protein expression analysis in blood neutrophils-CD16A

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Strain specific CD16A expression analysis in wild-type CB-17 SCID mice and homozygous B-hCD16A/hNKP46 mice(CB-17 SCID) by flow cytometry. Blood were collected from wild-type CB-17 SCID mice (+/+) and homozygous B-hCD16A/hNKP46 mice(CB-17 SCID) (H/H) (n=3, 6-week old). Protein expression was analyzed with anti-mouse CD16.2 (FcγRIV) antibody (Biolegend, 149503) and anti-human CD16 antibody (Biolegend, 302008) by flow cytometry. Mouse CD16A was detectable in wild-type CB-17 SCID mice. Human CD16A was not detectable in homozygous B-hCD16A/hNKP46 mice(CB-17 SCID).

Protein expression analysis in bone marrow NK cells-CD16A

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Strain specific CD16A expression analysis in wild-type CB-17 SCID mice and homozygous B-hCD16A/hNKP46 mice(CB-17 SCID) by flow cytometry. Bone marrow were collected from wild-type CB-17 SCID mice (+/+) and homozygous B-hCD16A/hNKP46 mice(CB-17 SCID) (H/H) (n=3, 6-week old). Protein expression was analyzed with anti-mouse CD16.2 (FcγRIV) antibody (Biolegend, 149503) and anti-human CD16 antibody (Biolegend, 302008) by flow cytometry. Mouse CD16A was not detectable in wild-type CB-17 SCID mice. Human CD16A was exclusively detectable in homozygous B-hCD16A/hNKP46 mice(CB-17 SCID), but not in wild-type CB-17 SCID mice.

Protein expression analysis in bone marrow DC cells-CD16A

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Strain specific CD16A expression analysis in wild-type CB-17 SCID mice and homozygous B-hCD16A/hNKP46 mice(CB-17 SCID) by flow cytometry. Bone marrow were collected from wild-type CB-17 SCID mice (+/+) and homozygous B-hCD16A/hNKP46 mice(CB-17 SCID) (H/H) (n=3, 6-week old). Protein expression was analyzed with anti-mouse CD16.2 (FcγRIV) antibody (Biolegend, 149503) and anti-human CD16 antibody (Biolegend, 302008) by flow cytometry. Mouse CD16A was weakly detectable in wild-type CB-17 SCID mice. Human CD16A was weakly detectable in homozygous B-hCD16A/hNKP46 mice(CB-17 SCID).

Protein expression analysis in bone marrow monocytes-CD16A

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Strain specific CD16A expression analysis in wild-type CB-17 SCID mice and homozygous B-hCD16A/hNKP46 mice(CB-17 SCID) by flow cytometry. Bone marrow were collected from wild-type CB-17 SCID mice (+/+) and homozygous B-hCD16A/hNKP46 mice(CB-17 SCID) (H/H) (n=3, 6-week old). Protein expression was analyzed with anti-mouse CD16.2 (FcγRIV) antibody (Biolegend, 149503) and anti-human CD16 antibody (Biolegend, 302008) by flow cytometry. Mouse CD16A was detectable in wild-type CB-17 SCID mice. Human CD16A was exclusively detectable in homozygous B-hCD16A/hNKP46 mice(CB-17 SCID), but not in wild-type CB-17 SCID mice.

Protein expression analysis in bone marrow macrophages-CD16A

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Protein expression analysis in bone marrow neutrophils-CD16A

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Strain specific CD16A expression analysis in wild-type CB-17 SCID mice and homozygous B-hCD16A/hNKP46 mice(CB-17 SCID) by flow cytometry. Bone marrow were collected from wild-type CB-17 SCID mice (+/+) and homozygous B-hCD16A/hNKP46 mice(CB-17 SCID) (H/H) (n=3, 6-week old). Protein expression was analyzed with anti-mouse CD16.2 (FcγRIV) antibody (Biolegend, 149503) and anti-human CD16 antibody (Biolegend, 302008) by flow cytometry. Mouse CD16A was detectable in wild-type CB-17 SCID mice. Human CD16A was not detectable in homozygous B-hCD16A/hNKP46 mice(CB-17 SCID).

Frequency of leukocyte subpopulations in spleen, blood and bone marrow

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Frequency of leukocyte subpopulations in spleen by flow cytometry. Splenocytes (A), blood (B) and bone marrow (C) were isolated from wild-type CB-17 SCID mice and homozygous B-hCD16A/hNKP46 mice(CB-17 SCID) (n=3, 7-week-old). Flow cytometry analysis was performed to assess the frequency of leukocyte subpopulations. Percentages of NK cells, DCs, monocytes, macrophages and neutrophils in B-hCD16A/hNKP46 mice(CB-17 SCID) were similar to those in CB-17 SCID mice, demonstrating that humanization of CD16A and NKP46 does not change the frequency or distribution of these cell types in spleen, blood and bone marrow. Values are expressed as mean ± SEM. Significance was determined by two-way ANOVA test. *P < 0.05, **P < 0.01, ***P < 0.001.