Strain specific analysis of Abcg2 mRNA expression in wild-type C57BL/6 mice and B-Bcrp KO mice by RT-PCR. Brain and kidney RNA were isolated from wild-type C57BL/6 mice (+/+) and B-Bcrp KO mice (-/-), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse Abcg2 primers. Mouse Abcg2 mRNA was detectable in wild-type C57BL/6 mice (+/+) but not in B-Bcrp KO mice (-/-).

Western blot analysis of BCRP protein expression in B-Bcrp KO mice. Various tissue lysates were collected from wild-type C57BL/6 mice (+/+) and B-Bcrp KO mice (-/-), and then analyzed by western blot with anti-BCRP antibody. 40 μg total proteins were loaded for western blotting analysis. BCRP was detectable in kidney, liver, heart of wild-type C57BL/6 mice (+/+) but not B-Bcrp KO mice (-/-).