B-FcRn KO mice_Biocytogen Pharmaceuticals (Beijing) Co., Ltd._Biocytogen, drug development, antibody discovery

Strain Name	C57BL/6N-Fcgrt^tm1Bcgen/Bcgen	Common Name	B-FcRn KO mice
Background	C57BL/6N	Catalog number	110165
Related Genes	FCGRT(Fc fragment of IgG receptor and transporter)
NCBI Gene ID	14132

mRNA expression analysis

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Strain specific analysis of Fcgrt gene expression in C57BL/6(+/+) and B-FcRn KO mice by RT-PCR.

Kidney was collected from C57BL/6(+/+) mice and homozygous B-FcRn KO mice. Mouse Fcgrt mRNA was detectable in C57BL/6(+/+) mice but not the homozygous B-FcRn KO mice.

Protein expression analysis

Strain specific analysis of FcRn expression in C57BL/6(+/+) and the homozygous B-FcRn KO mice by western blot.

Kidney was collected from C57BL/6(+/+) mice and homozygous B-FcRn KO mice. Mouse FcRn was detectable in C57BL/6(+/+) mice but not the homozygous B-FcRn KO mice.

FcRn-deficient mice have low serum IgG

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Sera from C57BL/6(+/+) mice and B-FcRn KO mice were analyzed by ELISA .