| Strain Name |
|
Stock No. | 210488 |
| Common name |
B-Drd2-Cre rats |
Gene symbol and name | Drd2 (dopamine receptor D2), D2R; Drd-2 |
| Strain of origin |
SD |
Chromosome | 8 |
| Coat color | White | Dietary information | Growth and reproduction diet for experimental mice |
|
NCBI Gene ID |
24318 | ||
Application
- Function research of genes
- This Drd2Cre model is an efficient tool to study various gene functions when crossed with rats with different loxP site-flanked genes of interest, especially in studies of D2 dopamine receptor subtype-expressing neurons, and may be useful for studying dopamine-sensitive behaviors such as addiction.
Expression
Expressed gene
Cre, cre recombinase, bacteriophage P1
Site of expression
postsynaptic dopaminergic neurons
Gene editing strategy
A P2A-NLS-Cre sequence cassette was placed between the coding sequence of exon 3 and 3’UTR of the Drd2 gene. This strain was maintained on a Sprague Dawley genetic background.
Phenotype Analysis
k Immunofluorescent images of tdTomato and DRD2 in the striatum of Drd2 reporter rats. Arrows indicate the colocalization of tdTomato and DRD2. Scale bar, 25 µm. l Western blot of DRD2 and GAPDH in the striatum from Drd2::Cre+/− and WT rats. Left, representative blots, right, quantifcation results. NS not signifcant, n= 3, t test
(source: Yu Q et al.,2019)
reference
1. Yu Q, Liu YZ, Zhu YB, Wang YY, Li Q, Yin DM. Genetic labeling
reveals temporal and spatial expression pattern of D2 dopamine receptor in rat
forebrain. Brain Struct Funct. 2019 Apr;224(3):1035-1049. [PubMed: 30604007]
2. Bunzow, J. R., Van Tol, H. H., Grandy, D. K., Albert, P.,
Salon, J., Christie, M., Machida, C. A., Neve, K. A., Civelli, O. Cloning and expression
of a rat D2 dopamine receptor cDNA. Nature 336: 783-787, 1988. [PubMed:
2974511]
3. Duan, J., Wainwright, M. S., Comeron, J. M., Saitou, N.,
Sanders, A. R., Gelernter, J., Gejman, P. V. Synonymous mutations in the human
dopamine receptor D2 (DRD2) affect mRNA stability and synthesis of the
receptor. Hum. Molec. Genet. 12: 205-216, 2003. [PubMed: 12554675]
4. Basu, S., Nagy, J. A., Pal, S., Vasile, E., Eckelhoefer, I.
A., Bliss, V. S., Manseau, E. J., Dasgupta, P. S., Dvorak, H. F., Mukhopadhyay,
D. The neurotransmitter dopamine inhibits angiogenesis induced by vascular
permeability factor/vascular endothelial growth factor. Nature Med. 7:
569-574, 2001. [PubMed: 11329058]
