| Strain Name |
|
Stock No. | 110139 |
| Common name |
B-Htr2c-iCre mice |
Gene symbol and name | Htr2c(5-hydroxytryptamine (serotonin) receptor 2C), SR1; Htr1; 5HT1c |
| Strain of origin |
C57BL/6 |
Chromosome | X |
| Coat color | Black | Dietary information | Growth and reproduction diet for experimental mice |
|
NCBI Gene ID |
15560 | ||
Application
- Function research of genes
- This Htr2ciCre model is an efficient tool to study the various gene functions when crossed with mice with different loxP site-flanked genes of interest.
Expression
Expressed gene
iCre, improved cre recombinase, bacteriophage P1
Site of expression
Biased expression in cerebellum adult, frontal lobe adult.
Gene editing strategy
An P2A-iCre sequence cassette was placed between the coding sequence of exon 6 and 3’UTR of the Htr2c gene. This strain was maintained on a C57BL/6 genetic background.
Phenotype Analysis
Top: a construct was used to generate CaMPARIloxP/loxP mice.
Bottom: low-and high-magnifications images show
CaMPARI expression in Htr2c cells after crossing CaMPARIloxP/loxP mice with Htr2c-Cre mice.
reference
1. Li X, Chen W, Pan K, Li H, Pang P, Guo Y, Shu S, Cai Y, Pei
L, Liu D, Afewerky HK, Tian Q, Zhu LQ, Lu Y. Serotonin receptor 2c-expressing
cells in the ventral CA1 control attention via innervation of the
Edinger-Westphal nucleus. Nat Neurosci. 2018 Sep;21(9):1239-1250. [PubMed: 30104733]
2. Burke LK, Doslikova B, D'Agostino G, Greenwald-Yarnell M,
Georgescu T, Chianese R, Martinez de Morentin PB, Ogunnowo-Bada E, Cansell C,
Valencia-Torres L, Garfield AS, Apergis-Schoute J, Lam DD, Speakman JR,
Rubinstein M, Low MJ, Rochford JJ, Myers MG, Evans ML, Heisler LK. Sex
difference in physical activity, energy expenditure and obesity driven by a
subpopulation of hypothalamic POMC neurons. Mol Metab. 2016 Jan
22;5(3):245-252. [PubMed: 26977396]
3. Park S, Williams KW, Liu C, Sohn JW. A neural basis for tonic
suppression of sodium appetite. Nat Neurosci. 2020 Mar;23(3):423-432. [PubMed: 31959933]
