| Strain Name |
|
Stock No. | 110127 |
| Common name |
B-Nkp46-iCre mice |
Gene symbol and name | Ncr1(natural cytotoxicity triggering receptor 1), NKp46; Ly94 |
| Strain of origin |
C57BL/6 |
Chromosome | 7 |
| Coat color | Black | Dietary information | Growth and reproduction diet for experimental mice |
|
NCBI Gene ID |
17086 | ||
Application
- Function research of genes
- This Ncr1iCre model is an efficient tool to study various gene functions when crossed with mice with different loxP site-flanked genes of interest, especially in studies of natural killer (NK) cells related signaling.
Expression
Expressed gene
iCre, improved cre recombinase, bacteriophage P1
Site of expression
The molecular is expressed on resting and activated NK cells
Gene editing strategy
An IRES-iCre sequence cassette was placed between the coding sequence of exon 7 and 3’UTR of the Nkp46 gene using C57BL/6 embryonic stem cells. Neo cassette flanked by frt sites was inserted at least 300bp downstream of 3’UTR. This strain was maintained on a C57BL/6 genetic background.
Phenotype Analysis
NKp46 þ ILC3
cells from Washflox/flox and Washflox/floxNcr1-Cre
mice were in
situ hybridized with probes
against Ahr promoter, followed by staining with antibodies
against WASH and Arid1a (upper panel). White arrow head indicates Ahr promoters colocalized with
Arid1a. Percentages of cells with Arid1a colocalized Ahr promoter were
calculated (lower panel). At least 200 NKp46 þ ILC3 cells were counted. Scale bar, 5 mm.
(source: Xia P et al.,2017)
reference
1. Xia P, Liu J, Wang S, Ye B, Du Y, Xiong Z, Han ZG, Tong L,
Fan Z. WASH maintains NKp46+ ILC3 cells by promoting AHR expression. Nat Commun.
2017 Jun 7;8:15685. [PubMed: 28589939]
2. Wang S, Xia
P, Huang G, Zhu P, Liu J, Ye B, Du Y, Fan Z. FoxO1-mediated autophagy is
required for NK cell development and innate immunity. Nat Commun. 2016 Mar
24;7:11023. [PubMed: 27010363]
3. Elboim M; Gazit R; Gur C; Ghadially H; Betser-Cohen G;
Mandelboim O. 2010. Tumor immunoediting by NKp46. J Immunol 184(10):5637-44.
[PubMed:20404273]
4. Ghadially H; Horani A; Glasner A; Elboim M; Gazit R; Shoseyov
D; Mandelboim O. 2013. NKp46 regulates allergic responses. Eur J Immunol
43(11):3006-16. [PubMed: 23878025]
5. Ghadially H; Ohana M; Elboim M; Gazit R; Gur C; Nagler A;
Mandelboim O. 2014. NK Cell Receptor NKp46 Regulates Graft-versus-Host Disease.
Cell Rep 7(6):1809-14. [PubMed: 24882008]
6. Glasner A; Ghadially H; Gur C; Stanietsky N; Tsukerman P; Enk
J; Mandelboim O. 2012. Recognition and Prevention of Tumor Metastasis by the NK
Receptor NKp46/NCR1. J Immunol 188(6):2509-15. [PubMed: 22308311]
7. Gur C; Porgador A; Elboim M; Gazit R; Mizrahi S;
Stern-Ginossar N; Achdout H; Ghadially H; Dor Y; Nir T; Doviner V; Hershkovitz
O; Mendelson M; Naparstek Y; Mandelboim O. 2010. The activating receptor NKp46
is essential for the development of type 1 diabetes. Nat Immunol 11(2):121-8.
[PubMed: 20023661]
