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B-Oxt-iCre rats
Strain Name

SD-Oxttm1(icre)Bcgen/Bcgen

Stock No.  210501
Common name

B-Oxt-iCre rats

Gene symbol and name  Oxt (oxytocin), OT, Ox, Oxy
Strain of origin

SD

Chromosome  2
Coat color White Dietary information Growth and reproduction diet for experimental mice
NCBI Gene ID
18429

Application

  • Function research of genes
  • This OxtiCre model is an efficient tool to study various gene functions when crossed with rats with different loxP site-flanked genes of interest, especially in studies of Oxytocin expressed in the PVN, SON and the olfactory cue. The development of social familiarity in rodents depends predominantly on olfactory cues and can critically influence reproductive success.

Expression


Expressed gene

iCre, improved cre recombinase, bacteriophage P1


Site of expression

supraoptic nucleus, paraventricular nuclei, the magnocellular subnuclei of PVN


Gene editing strategy


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A P2A-iCre sequence cassette was inserted upstream 3’UTR of the Oxt gene. This strain was maintained on a Sprague Dawley genetic background.


Phenotype Analysis


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(A) Schematic of AAV-Retro-DIO-YFP viral injection in OXT-Cre rats.

(B) Immunostaining of YFP with OXT-NP on viral injected OXT-Cre rat brain slices. Co-localization of YFP with OXT-NP is indicated with white triangles.

(C) Parvocellular neurons in the PVN were negative for YFP.

(J) Schematic injection and chemogenetic inhibition of Magno-OXT neurons with AAV-Retro-DIO-hM4Di.

(K) Representative images showing the viral infection of Magno-OXT neurons. Co-localization of mCherry with OXT-NP is indicated with white triangles.                               

 (source: Zhang B et al.,2021)

reference


1. Zhang B, Qiu L, Xiao W, Ni H, Chen L, Wang F, Mai W, Wu J, Bao A, Hu H, Gong H, Duan S, Li A, Gao Z. Reconstruction of the Hypothalamo-Neurohypophysial System and Functional Dissection of Magnocellular Oxytocin Neurons in the Brain. Neuron. 2021 Jan 20;109(2):331-346.e7. [PubMed: 33212012]

2. Armstrong WE, Warach S, Hatton GI, McNeill TH. Subnuclei in the rat hypothalamic paraventricular nucleus: a cytoarchitectural, horseradish peroxidase and immunocytochemical analysis. Neuroscience. 1980;5(11):1931-58. [PubMed: 7432630]