| Strain Name |
|
Stock No. | 210501 |
| Common name |
B-Oxt-iCre rats |
Gene symbol and name | Oxt (oxytocin), OT, Ox, Oxy |
| Strain of origin |
SD |
Chromosome | 2 |
| Coat color | White | Dietary information | Growth and reproduction diet for experimental mice |
|
NCBI Gene ID |
18429 | ||
Application
- Function research of genes
- This OxtiCre model is an efficient tool to study various gene functions when crossed with rats with different loxP site-flanked genes of interest, especially in studies of Oxytocin expressed in the PVN, SON and the olfactory cue. The development of social familiarity in rodents depends predominantly on olfactory cues and can critically influence reproductive success.
Expression
Expressed gene
iCre, improved cre recombinase, bacteriophage P1
Site of expression
supraoptic nucleus, paraventricular nuclei, the magnocellular subnuclei of PVN
Gene editing strategy
A P2A-iCre sequence cassette was inserted upstream 3’UTR of the Oxt gene. This strain was maintained on a Sprague Dawley genetic background.
Phenotype Analysis
(A)
Schematic of AAV-Retro-DIO-YFP viral injection in OXT-Cre rats.
(B)
Immunostaining of YFP with OXT-NP on viral injected OXT-Cre rat brain slices.
Co-localization of YFP with OXT-NP is indicated with white triangles.
(C)
Parvocellular neurons in the PVN were negative for YFP.
(J) Schematic injection and
chemogenetic inhibition of Magno-OXT neurons with AAV-Retro-DIO-hM4Di.
(K) Representative images showing the viral infection of Magno-OXT neurons. Co-localization of mCherry with OXT-NP is indicated with white triangles.
(source: Zhang B et al.,2021)
reference
1. Zhang B, Qiu L, Xiao W, Ni H, Chen L, Wang F, Mai W, Wu J,
Bao A, Hu H, Gong H, Duan S, Li A, Gao Z. Reconstruction of the
Hypothalamo-Neurohypophysial System and Functional Dissection of Magnocellular
Oxytocin Neurons in the Brain. Neuron. 2021 Jan 20;109(2):331-346.e7. [PubMed: 33212012]
