B-CAG-Upar-tdTomato cKI mice_Biocytogen Pharmaceuticals (Beijing) Co., Ltd._Biocytogen, drug development, antibody discovery

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B-CAG-Upar-tdTomato cKI mice

Strain Name	C57BL/6-Gt(ROSA)26Sor^{tm1(CAG-LSL-mUPAR-IRES2-tdTomato)Bcgen}/Bcgen	Stock No.	111205
Common name	B-CAG-Upar-tdTomato cKI mice	Gene symbol and name	Tgfb1(transforming growth factor, beta 1), Tgfb
Strain of origin	C57BL/6	Chromosome	6
Coat color	Black	Dietary information	Growth and reproduction diet for experimental mice

Description

ROSAS26 gene produces a long non-coding RNA (lncRNA) that is under the control of a constitutive promoter. This locus is a widely used site for the integration of transgenes and reporter constructs

Application

B-CAG-Upar-tdTomato cKI mice model is an efficient tool to study the mouse UPAR function in different tissues after cross with the tissue specification Cre mice.

Targeting strategy

Gene targeting strategy. The CAG-LSL-mUPAR-IRES2-tdTomato-WPRE-pA cassette that containing the full-length coding sequence of mouse Upar gene as well as tdTomato coding sequence were inserted into mouse Rosa26 gene locus site. The targeted mice crossed with Cre-expressing mice to implement tissue-specific expression.

Protein expression analysis

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Mouse UPAR expression analysis in B-CAG-Upar-tdTomato cKI mice by flow cytometry. The B-CAG-Upar-tdTomato cKI mice were cross breeding with CMV-Cre mice, which is useful for deletion of loxP-flanked genes in all tissues. The splenocytes were collected from the offspring mice in UPAR (△/+) Cre (Tg/+) genotype, and analyzed by flow cytometry with anti-mouse UPAR antibody. As shown, the mUPAR was detectable in offspring mice. In addition, the tdTomato will co-expression as a reporter.