• B-Lyz2-EGFP-DTR-luciferase mice have the mouse Lyz2 promoter to determine the expression of DTR (Diphtheria toxin receptor), EGFP (enhanced green fluorescent protein) and luciferase. 
• Neutrophils, monocytes and macrophages will be traced by EGFP or Luciferase reporting system in B-Lyz2-EGFP-DTR-luciferase mice. 
• Neutrophils, monocytes and macrophages will be depleted in B-Lyz2-EGFP-DTR-luciferase mice injected with DT (diphtheria toxin). 
• The mice can be used to study the specific role of neutrophils, monocytes and macrophages in immune response.

Frequency of leukocyte subpopulations in spleen by flow cytometry. Splenocytes were isolated from wild-type C57BL/6 mice (+/+) and heterozygous B-Lyz2-EGFP-DTR-luciferase mice (Mut/+) (Male, n=3, 8-week-old) injected with PBS or DT (50 ng/g body weight) for two consecutive days. Flow cytometry analysis of the splenocytes was performed to assess the frequency of leukocyte subpopulations. Values are expressed as mean ± SEM.

Frequencies of CD11b+ EGFP+ cells and EGFP+ cells in spleen by flow cytometry. Splenocytes were isolated from wild-type C57BL/6 mice (+/+) and heterozygous B-Lyz2-EGFP-DTR-luciferase mice (Mut/+) (Male, n=3, 8-week-old) injected with PBS or DT (50 ng/g body weight) for two consecutive days. Flow cytometry analysis of the splenocytes was performed to assess the frequencies of CD11b+ EGFP+ cells (A) and EGFP+ cells (B) in total CD45+ cells. The frequencies of EGFP+ cells (A) and CD11b+ EGFP+ cells (B) were decreased in heterozygous mice injected with DT.