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B-luc B16-F10
Common name

 B-luc B16-F10

Catalog number    310680
Aliases

NA

Disease  Melanoma
Organism

Mouse

Strain  C57BL/6
Tissue types


Skin


Tissue Skin

Description 


This B-luc B16-F10 cell line expresses firefly luciferase as a marker of B16-F10 cells. Luminescence can be observed B-luc B16-F10 cells.

Application

B-luc B16-F10 cell line can be used to evaluate anti-cancer drugs in melanoma in vivo.



Gene targeting strategy


Gene targeting strategy for B-luc B16-F10 . The luciferase cDNA sequence with CAG promoter was inserted in the ROSA26 exon1 of  wild-type B16-F10.


In vitro luciferase activity of B-luc B16-F10 cells 


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Luminescence signal intensity of B-luc B16-F10 cells. Luminescence intensity was measured using the Bright-GloTM luciferase Assay (Promega, Cat E2610). B-luc B16-F10 cells have a strong luminescence signal. The C5 clone of B-luc B16-F10 cells can be used in vivo experiments.

Tumor growth curve & Body weight changes

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Subcutaneous homograft tumor growth of B-luc B16-F10 cells. B-luc B16-F10 cells and wild-type B16-F10 cells were subcutaneously implanted into C57BL/6 mice (female, 7-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume. (B)  Body weight. Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-luc B16-F10 cells were able to establish tumors in vivo and can be used for efficacy studies. Values are expressed as mean ± SEM.


Quantitative imaging data & body weight changes


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Tumor growth and in vivo imaging of B-luc B16-F10 cells. B-luc B16-F10 cells (1x105 and 2x105) were subcutaneously implanted into the wild-type C57BL/6 mice. Signal intensity and body weight were measured twice a week. (A) Imaging was performed twice a week. (B) Body weight (Mean ± SEM). B-luc B16-F10 cells can be used for in vivo efficacy evaluation.