B-Luc-EGFP HL-60_Biocytogen Pharmaceuticals (Beijing) Co., Ltd._Biocytogen, drug development, antibody discovery

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B-Luc-EGFP HL-60

Common name	B-Luc-EGFP HL-60	Catalog number	321821
Aliases	NA	Disease	Acute myelogenous leukemia
Organism	Human	Strain	NA
Tissue types	Peripheral blood	Tissue	Peripheral blood

Description

This B-Luc-EGFP HL-60 cell line expresses firefly luciferase as a marker of HL-60 cells. Fluorescence could be detected in B-Luc-EGFP HL-60 cells.

Application

B-Luc-EGFP HL-60 cell line can be used to evaluate anti-cancer drugs in acute myelogenous leukemia in vivo.

Targeting strategy

Gene targeting strategy for B-Luc-EGFP HL-60 cells. The CMV promoter and luciferase-EGFP were inserted into the targeting vector used in the lentivirus system.

In vitro luciferase activity of B-Luc-EGFP HL-60 cells

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Luminescence signal intensity of B-Luc-EGFP HL-60 cells. Luminescence intensity was measured using the Bright-GloTM luciferase Assay (Promega, Cat E2610). B-Luc-EGFP HL-60 cells have a strong luminescence signal. EGFP was detected in B-Luc-EGFP HL-60 cells. The 1-B06 clone of B-Luc-EGFP HL-60 cells can be used in vivo experiments.

Quantitative imaging data & body weight changes

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B-Luc-EGFP HL-60 cells (1x10⁵) were injected into the tail vein of wild-type B-NDG mice. Signal intensity and body weight were measured twice a week. (A) Imaging was performed twice a week. (B) Body weight (Mean ± SEM). B-Luc-EGFP HL-60 cells can be used for in vivo efficacy evaluation.