Luminescence signal intensity of B-Luc Hep 3B plus cells. Luminescence intensity was measured using the Bright-GloTM luciferase Assay (Promega, Cat E2610). B-Luc Hep 3B plus cells have a strong luminescence signal. The 1-A06 clone of B-Luc Hep 3B plus cells can be used in vivo experiments.

Subcutaneous homograft tumor growth of B-Luc Hep 3B plus cells. B-Luc Hep 3B plus cells (8x10⁶ ) and wild-type Hep 3B cells (8x10⁶) were subcutaneously implanted into b-NDG mice (female, 5-week-old, n=4). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B-Luc Hep 3B plus cells were able to establish tumors in vivo and can be used for efficacy studies.

Tumor growth and in vivo imaging of B-Luc Hep 3B plus cells. B-Luc Hep 3B plus cells (8x10⁶) were subcutaneously implanted into the wild-type B-NDG mice. Signal intensity and body weight were measured twice a week. (A) Imaging was performed twice a week. (B) Body weight (Mean ± SEM). B-Luc Hep 3B plus cells can be used for in vivo efficacy evaluation.