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B-Luc LLC1
Common name

 B-Luc LLC1

Catalog number    321857
Aliases

NA

Disease  Lewis lung carcinoma
Organism
Mouse Strain  C57BL
Tissue types

Lung

Tissue  Lung

Description 


This B-Luc LLC1 cell line expresses firefly luciferase as a marker of LLC1 cells. Luminescence can be observed in B-Luc LLC1 cells.


Application

B-Luc-EGFP HL-60 cell line can be used to evaluate anti-cancer drugs in acute myelogenous leukemia B-Luc LLC1 cell line can be used to evaluate anti-cancer drugs in Lewis lung carcinoma in vivo.

Targeting strategy

Gene targeting strategy for B-Luc LLC1 cells. The exogenous promoter and luciferase was inserted into the ROSA26 locus in murine.
 

In vitro luciferase activity of B-Luc LLC1 cells

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Luminescence signal intensity of B-Luc LLC1 cells. Luminescence intensity was measured using the Bright-GloTM luciferase Assay (Promega, Cat E2610). B-Luc LLC1 cells have a strong luminescence signal. The 1-H04 clone of B-Luc LLC1 cells can be used in vivo experiments.


Tumor growth curve & Body weight changes

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Subcutaneous homograft tumor growth of B-Luc LLC1 cells. B-Luc LLC1 cells (1x105 and 2x105) and wild-type LLC1 cells (1x105) were subcutaneously implanted into C57BL/6 mice (female, 9-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-Luc LLC1 cells were able to establish tumors in vivo and can be used for efficacy studies.


Quantitative imaging data & body weight changes

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Tumor growth and in vivo imaging of B-Luc LLC1 cells. B-Luc LLC1 cells (1x105 and 2x105) were subcutaneously implanted into the wild-type C57BL/6 mice. Signal intensity and body weight were measured twice a week. (A) Imaging was performed twice a week. (B) Body weight (Mean ± SEM). B-Luc LLC1 cells can be used for in vivo efficacy evaluation.

Imaging analysis in vivo



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In vivo luciferase images of B-Luc LLC1 (C57BL/6, B-Luc LLC1, 1x105, s.c.)