Common name
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B-Luc THP-1
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Catalog number
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321919
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Aliases
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NA
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Disease
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Acute monocytic leukemia
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Organism
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Human
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Strain
|
NA
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Tissue types
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Peripheral blood
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Tissue
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Peripheral blood
|
Description
This B-Luc THP-1 cell line expresses firefly luciferase as a marker of THP-1 cells. Fluorescence could be detected in B-Luc THP-1 cells.
Application
B-Luc THP-1 cell line can be used to evaluate anti-cancer drugs in acute monocytic leukemia
in vivo.
Targeting strategy
Gene targeting strategy for B-Luc THP-1 cells. The CMV promoter and luciferase were inserted into the targeting vector used in the lentivirus system.
In vitro luciferase activity of B-Luc THP-1 cells
Luminescence signal intensity of B-Luc THP-1 cells. Luminescence intensity was measured using the Bright-GloTM luciferase Assay (Promega, Cat E2610). B-Luc THP-1 cells have a strong luminescence signal. The 1-D09 clone of B-Luc THP-1 cells can be used in vivo experiments.
Quantitative imaging data & body weight changes
Tumor growth and in vivo imaging of B-Luc THP-1 cells. B-Luc THP-1 cells (1x106) were injected into the tail vein of wild-type B-NDG mice. Signal intensity and body weight were measured twice a week. (A) Imaging was performed twice a week. (B) Body weight (Mean ± SEM). B-Luc THP-1 cells can be used for in vivo efficacy evaluation.
Imaging analysis in vivo