B-Tg(Luc) RKO_Biocytogen Pharmaceuticals (Beijing) Co., Ltd._Biocytogen, drug development, antibody discovery

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B-Tg(Luc) RKO

Common name	B-Tg(Luc) RKO	Catalog number	321871
Aliases	NA	Disease	Colon carcinoma
Organism	Human	Strain	NA
Tissue types	Colon	Tissue	Colon

Description

This B-Tg(Luc) RKO cell line expresses firefly luciferase as a marker of RKO cells. Fluorescence could be detected in B-Tg(Luc) RKO cells.

Application

B-Tg(Luc) RKO cell line can be used to evaluate anti-cancer drugs in colon cancer in vivo. It can be applied subcutaneously and orthotopically to colons in mice

Targeting strategy

Gene targeting strategy for B-Tg(Luc) RKO cells. The exogenous promoter and luciferase coding sequence were inserted into the mouse genome randomly.

In vitro luciferase activity of B-Tg(Luc) RKO cells

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Luminescence signal intensity of B-Tg(Luc) RKO cells. Luminescence intensity was measured using the Bright-GloTM luciferase Assay (Promega, Cat E2610). B-Tg(Luc) RKO cells have a strong luminescence signal. The mix clone of B-Tg(Luc) RKO cells can be used for in vivo experiments.

Quantitative imaging data & body weight changes

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B-Tg(Luc) RKO cells (5x10⁵ ,1x10⁶) were injected into the colon of wild-type B-NDG mice. Signal intensity and body weight were measured twice a week. (A) Imaging was performed twice a week. (B) Body weight (Mean ± SEM). B-Tg(Luc) RKO cells can be used for in vivo efficacy evaluation.

Imaging analysis in vivo

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HE staining

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Tumor metastasis occurred in the liver of mice by HE.

Tumor growth curve & Body weight changes

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Subcutaneous homograft tumor growth of B-Tg(Luc) RKO cells. B-Tg(Luc) RKO cells (5x10⁶) were subcutaneously implanted into B-NDG mice (female, 7-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B-Tg(Luc) RKO cells were able to establish tumors in vivo and can be used for efficacy studies.