B-luc A20_Biocytogen Pharmaceuticals (Beijing) Co., Ltd._Biocytogen, drug development, antibody discovery

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B-luc A20

Common name	B-Luc A20	Catalog number	311118
Aliases	Gt(ROSA)26Sor	Disease	Reticulum cell sarcoma
Organism	Mouse	Strain	BALB/cAnN
Tissue types	Lymphoma	Tissue	B lymphocyte

Description

This B-Luc A20 cell line expresses firefly luciferase as a marker of A20 cells. Fluorescence and luminescence can be observed in B-Luc A20 cells.

Application

B-Luc A20 cell line can be used to evaluate anti-cancer drugs in reticulum cell sarcoma in vivo.

Targeting strategy

Gene targeting strategy for B-Luc A20 cells. The exogenous promoter and luciferase was inserted into the ROSA26 locus in murine.

In vitro luciferase activity of B-Luc A20 cells

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Luminescence signal intensity of B-Luc A20 cells. Luminescence intensity was measured using the Bright-Glo^TM luciferase Assay (Promega, Cat E2610). B-Luc A20 cells have a strong luminescence signal. Clone No. :2-A10.

Quantitative imaging data & body weight changes

Tumor growth and in vivo imaging of B-Luc A20 cells. B-Luc A20 cells (5x10⁵) were injected into the tail vein of wild-type BALB/c mice. Signal intensity and body weight were measured twice a week. (A) Imaging was performed twice a week. (B) Body weight (Mean ± SEM). B-Luc A20 cells can be used for in vivo efficacy evaluation.

Imaging analysis in vivo