B-hALB MC38_Biocytogen Pharmaceuticals (Beijing) Co., Ltd._Biocytogen, drug development, antibody discovery

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B-hALB MC38

Common name	B-hALB MC38	Catalog number	321909
Aliases	HSA; PRO0883; PRO0903; PRO1341	Disease	Colon carcinoma
Organism	Mouse	Strain	C57BL/6
Tissue types	Colon	Tissue	Colon

Description

The mouse Alb gene was replaced by human ALB coding sequence in B-hALB MC38 cells. Human ALB can be secreted by B-hALB MC38 cells.

Application

B-hALB MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.

Targeting strategy

Gene targeting strategy for B-hALB MC38 cells. The exogenous promoter and human ALB coding sequence was inserted to replace part of murine exon 1~2. The insertion disrupts the endogenous murine Alb gene, resulting in a non-functional transcript.

Protein expression analysis

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ALB expression analysis in B-hALB MC38 cells by ELISA. (A) Mouse ALB was detected in the supernatant of B-Tg(mAlb) MC38 cells but not wild-type MC38 cells. It has been verified that MC38 cells do not express the mouse ALB. (B) Human ALB was detected in the supernatant of B-hALB MC38 cells but not wild-type MC38 cells. The 3-B01 clone of B-hALB MC38 cells was used for in vivo experiments.

Tumor growth curve & Body weight changes

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Subcutaneous homograft tumor growth of B-hALB MC38 cells. B-hALB MC38 cells (1x10⁶) and wild-type MC38 cells (5x10⁵) were subcutaneously implanted into C57BL/6N mice (female, 6-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B-hALB MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.

Tumor growth curve of individual mice

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B-hALB MC38 tumor cells growth of individual mice. B-hALB MC38 cells (1x10⁶) and wild-type MC38 cells (5x10⁵) were subcutaneously implanted into C57BL/6N mice (female, 6-week-old, n=5). As shown in panel, B-hALB MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.

Protein expression analysis of tumor cells

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B-hALB MC38 cells were subcutaneously transplanted into C57BL/6N mice (n=5), and on 35 days post inoculation, tumor cells were harvested and assessed for mouse and human ALB expression by ELISA. As shown, human ALB was highly expressed tumor homogenate. Therefore, B-hALB MC38 cells can be used for in vivo efficacy studies of novel ALB therapeutics.