top
Please input keywords
Order
*Country
한국
미국
일본
중국
영국
호주
프랑스
독일
이탈리아
싱가포르
인도
러시아
캐나다
스위스
기타 국가
*Province
*City
*Name
*Telephone
*Company
*Position
*Email
*Verification code
*Verification Code
B-hCD37 MC38
Common name
B-hCD37 MC38 Catalog number    310976
Aliases GP52-40, TSPAN26 Disease  Colon carcinoma
Organism
Mouse
Strain  C57BL/6
Tissue types Colon Tissue  Colon

Description 


The CDS of human CD37 was inserted to replace part of murine exon 3 and exons 4-7 in B-hCD37 MC38 cells. Human CD37 is highly expressed on the surface of B-hCD37 MC38 cells.

Application

B-hCD37 MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.

Targeting strategy


Gene targeting strategy for B-hCD37 MC38 cells. The CDS of human CD37 was inserted to replace part of murine exon 3 and exons 4-7. The insertion disrupts the endogenous murine CD37 gene, resulting in a non-functional transcript.

Protein expression analysis


from clipboard

CD37 expression analysis in B-hCD37 MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hCD37 MC38 cultures were stained with species-specific anti-CD37 antibody. Human CD37 was detected on the surface of B-hCD37 MC38 cells but not wild-type MC38 cells. The 1-D11 clone of B-hCD37 MC38 cells was used for in vivo experiments.


Tumor growth curve & Body weight changes

from clipboard

Subcutaneous homograft tumor growth of B-hCD37 MC38 cells. B-hCD37 MC38 cells (2x105,  5x105, 3x106) and wild-type MC38 cells (5x105) were subcutaneously implanted into C57BL/6 mice (female, 8-week-old, n=8). Tumor volume and body weight were measured twice a week. (A) Average tumor volume. (B)  Body weight. Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hCD37 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies. Values are expressed as mean ± SEM.


Tumor growth curve & Body weight changes


from clipboard


Subcutaneous homograft tumor growth of B-hCD37 MC38 cells. B-hCD37 MC38 cells and wild-type MC38 cells were subcutaneously implanted into B-hCD3E mice (female, 7-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume. (B)  Body weight. Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hCD37 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies. Values are expressed as mean ± SEM.