B-hCD38 MC38_Biocytogen Pharmaceuticals (Beijing) Co., Ltd._Biocytogen, drug development, antibody discovery

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B-hCD38 MC38

Common name	B-hCD38 MC38	Catalog number	310730
Aliases	ADPRC1	Disease	Colon carcinoma
Organism	Mouse	Strain	C57BL/6
Tissue types	Colon	Tissue	Colon

Description

The mouse Cd38 gene was replaced by chimeric CD38 coding sequence in B-hCD38 MC38 cells. Human CD38 is highly expressed on the surface of B-hCD38 MC38 cells.

Application

B-hCD38 MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.

Targeting strategy

Gene targeting strategy for B-hCD38 MC38 cells. The exogenous promoter and chimeric CD38 coding sequence was inserted to replace part of murine exon 2 and all of exon 3. The insertion disrupts the endogenous murine Cd38 gene, resulting in a non-functional transcript.

Protein expression analysis

CD38 expression analysis in B-hCD38 MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hCD38 MC38 cultures were stained with species-specific anti-CD38 antibody. Mouse CD38 was detected on the surface of wild-type MC38 cells. Human CD38 was detected on the surface of B-hCD38 MC38 cells but not wild-type MC38 cells. The 4-F06 clone of B-hCD38 MC38 cells was used for in vivo experiments.

Tumor growth curve & Body weight changes

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Subcutaneous homograft tumor growth of B-hCD38 MC38 cells. B-hCD38 MC38 cells (5x10⁵) and wild-type MC38 cells (5x10⁵) were subcutaneously implanted into C57BL/6N mice (female, 7-8-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm³ using the formula: V=0.5 × long diameter × short diameter². As shown in panel A, B-hCD38 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.

Tumor growth curve & Body weight changes

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Subcutaneous homograft tumor growth of B-hCD38 MC38 cells. B-hCD38 MC38 cells (5x10⁵ or 1x10⁶) and wild-type MC38 cells (5x10⁵) were subcutaneously implanted into B-hCD16A mice (female, 6-7-week-old, n=8). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm³ using the formula: V=0.5 × long diameter × short diameter². As shown in panel A, B-hCD38 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.

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Subcutaneous homograft tumor growth of B-hCD38 MC38 cells. B-hCD38 MC38 cells and wild-type MC38 cells were subcutaneously implanted into B-h4-1BB mice (female, 7-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume. (B) Body weight. Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B-hCD38 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies. Values are expressed as mean ± SEM.