|
Common name |
B-hCD47 MC38 | Catalog number | 310695 |
| Aliases | IAP, MER6, OA3 | Disease | Colon carcinoma |
| Genotype | Homozygous | Culture properties | Adherent |
|
Organism |
Mouse |
Strain | C57BL/6 |
| Tissue types | Colon | Tissue | Colon |
Description
The mouse Cd47 gene was replaced by human CD47 coding sequence in B-hCD47 MC38 cells. Human CD47 is highly expressed on the surface of B-hCD47 MC38 cells.
Application
Gene targeting strategy for B-hCD47 MC38 cells. The exogenous promoter and human CD47 coding sequence was inserted to replace part of murine exon 2 and all of exon 3. The insertion disrupts the endogenous murine Cd47 gene, resulting in a non-functional transcript.
Tumor growth curve & Body weight changes
Subcutaneous homograft tumor growth of B-hCD47 MC38 cells. B-hCD47 MC38 cells (5x105, 1x106, 5x106) and wild-type MC38 cells (5x105) were subcutaneously implanted into B-hCD3E mice (female, 7-week-old). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hCD47 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.
Tumor growth curve & Body weight changes
Subcutaneous homograft tumor growth of B-hCD47 MC38 cells. B-hCD47 MC38 cells and wild-type MC38 cells were subcutaneously implanted into B-h4-1BB mice (female, 9-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume. (B) Body weight. Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hCD47 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies. Values are expressed as mean ± SEM.
