B-hCLDN6 MC38_Biocytogen Pharmaceuticals (Beijing) Co., Ltd._Biocytogen, drug development, antibody discovery

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B-hCLDN6 MC38

Common name	B-hCLDN6 MC38	Catalog number	311733
Aliases	/	Disease	Colon carcinoma
Organism	Mouse	Strain	C57BL/6
Tissue types	Colon	Tissue	Colon

Description

The human CLDN6 coding sequence was inserted to mouse Cldn6 exon 2. Human CLDN6 is highly expressed on the surface of B-hCLDN6 MC38 cells.

Application

B-hCLDN6 MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.

Targeting strategy

Gene targeting strategy for B-hCLDN6 MC38 cells. The exogenous promoter and human CLDN6 coding sequence was inserted to mouse Cldn6 exon 2 gene locus site. The insertion disrupts the endogenous murine Cldn6 gene, resulting in a non-functional transcript.

Protein expression analysis

CLDN6 expression analysis in B-hCLDN6 MC38 cells by flow cytometry. Single cell suspensions from B-hCLDN6 MC38 cultures were stained with species-specific anti-CLDN6 antibody. Human CLDN6 was detected on the surface of B-hCLDN6 MC38 cells but not wild-type MC38 cells. The 2-B06 clone of B-hCLDN6 MC38 cells was used for in vivo experiments.

Tumor growth curve & Body weight changes

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Subcutaneous homograft tumor growth of B-hCLDN6 MC38 cells. B-hCLDN6 MC38 cells (5x10⁵) and wild-type MC38 cells (5x10⁵) were subcutaneously implanted into C57BL/6 mice (female, 7-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B-hCLDN6 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.

Protein expression analysis of tumor cells

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B-hCLDN6 MC38 cells were subcutaneously transplanted into C57BL/6 mice (n=5) . At the end of the experiment, tumor cells were harvested and assessed for human CLDN6 expression by flow cytometry. As shown, human CLDN6 was highly expressed on the surface of tumor cells. Therefore, B-hCLDN6 MC38 cells can be used for in vivo efficacy studies of novel CLDN6 therapeutics.

Tumor growth curve & Body weight changes

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Subcutaneous homograft tumor growth of B-hCLDN6 MC38 cells. B-hCLDN6 MC38 cells and wild-type MC38 cells were subcutaneously implanted into B-h4-1BB mice (female, 6-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume. (B) Body weight. Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B-hCLDN6 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies. Values are expressed as mean ± SEM.