Common name
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B-hHER2 EL4
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Catalog number
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310724
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Aliases
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CD340, HER-2, ERBB2
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Disease
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Lymphoma
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Organism
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Mouse
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Strain
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C57BL/6N
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Tissue types
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Lymphoma
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Tissue
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T lymphocyte
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Description
The mouse Erbb2 gene was replaced by human ERBB2 coding sequence in B-hHER2 EL4 cells. Human HER2 is highly expressed on the surface of B-hHER2 EL4 cells.
Application
B-hHER2 EL4 cells have the capability to establish tumors in vivo and can be used for efficacy studies.
Targeting strategy
Gene targeting strategy for B-hHER2 EL4 cells. The exogenous promoter and human ERBB2 coding sequence was inserted to replace part of murine exon 2 and all of exons 3~7. The insertion disrupts the endogenous murine Erbb2 gene, resulting in a non-functional transcript.
Protein expression analysis
HER2 expression analysis in B-hHER2 EL4 cells by flow cytometry. Single cell suspensions from wild-type EL4 and B-hHER2 EL4 cultures were stained with species-specific anti-HER2 antibody. Human HER2 was detected on the surface of B-hHER2 EL4 cells but not wild-type EL4 cells. The 2-H01 clone of B-hHER2 EL4 cells was used for in vivo experiments.
Tumor growth curve & Body weight changes
Subcutaneous homograft tumor growth of B-hHER2 EL4 cells. B-hHER2 EL4 cells (2x105) and wild-type EL4 cells (2x105) were subcutaneously implanted into C57BL/6 mice (female, 6-9-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hHER2 EL4 cells were able to establish tumors in vivo and can be used for efficacy studies.
Animals with tumor volume over 3000mm3 in the control group were euthanized.
Tumor growth curve & Body weight changes
Subcutaneous homograft tumor growth of B-hHER2 EL4 cells. B-hHER2 EL4 cells and wild-type EL4 cells were subcutaneously implanted into B-h4-1BB mice (female, 8-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume. (B) Body weight. Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hHER2 EL4 cells were able to establish tumors in vivo and can be used for efficacy studies. Values are expressed as mean ± SEM.
