B-hLILRB4 MC38_Biocytogen Pharmaceuticals (Beijing) Co., Ltd._Biocytogen, drug development, antibody discovery

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B-hLILRB4 MC38

Common name	B-hLILRB4 MC38	Catalog number	310815
Aliases	ILT3, LIR5, CD85K, ILT-3, LIR-5	Disease	Colon carcinoma
Organism	Mouse	Strain	C57BL/6
Tissue types	Colon	Tissue	Colon

Description

The human LILRB4 is expressed on the surface of B-hLILRB4 MC38 cells.

Application

B-hLILRB4 MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.

Targeting strategy

Gene targeting strategy for B-hLILRB4 MC38 cells. The exogenous promoter and human LILRB4 coding sequence was inserted to murine rosa26 site.

Protein expression analysis

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LILRB4 expression analysis in B-hLILRB4 MC38 cells by flow cytometry. Single cell suspensions from B-hLILRB4 MC38 cultures were stained with species-specific anti-human LILRB4 antibody. Human LILRB4 was detected on the surface of B-hLILRB4 MC38 cells. The 5-C01 clone of B-hLILRB4 MC38 cells was used for in vivo experiments.

Tumor growth curve & Body weight changes

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Subcutaneous homograft tumor growth of B-hLILRB4 MC38 cells. B-hLILRB4 MC38 cells (5x10⁵) and wild-type MC38 cells (5x10⁵) were subcutaneously implanted into C57BL/6N mice (female, 7-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm³ using the formula: V=0.5×long diameter × short diameter². As shown in panel A, B-hLILRB4 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.

Tumor growth curve & Body weight changes

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Subcutaneous homograft tumor growth of B-hLILRB4 MC38 cells. B-hLILRB4 MC38 cells (5x10⁵, 1x10⁶, 5x10⁶) and wild-type MC38 cells (5x10⁵) were subcutaneously implanted into B-hCD3E mice (female, 9-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B-hLILRB4 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.

Tumor growth curve & Body weight changes

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Subcutaneous homograft tumor growth of B-hLILRB4 MC38 cells. B-hLILRB4 MC38 cells and wild-type MC38 cells were subcutaneously implanted into B-h4-1BB mice (female, 6-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume. (B) Body weight. Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B-hLILRB4 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies. Values are expressed as mean ± SEM.