B-hMMP-8 MC38_Biocytogen Pharmaceuticals (Beijing) Co., Ltd._Biocytogen, drug development, antibody discovery

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B-hMMP-8 MC38

Common name	B-hMMP-8 MC38	Catalog number	322005
Aliases	HNC; CLG1; MMP-8; PMNL-CL	Disease	Colon carcinoma
Organism	Mouse	Strain	C57BL/6
Tissue types	Colon	Tissue	Colon

Description

The mouse Mmp8 gene was replaced by human MMP-8 coding sequence in B-hMMP-8 MC38 cells. Human MMP-8 is highly expressed on the supernatant of B-hMMP-8 MC38 cells.

Application

B-hMMP-8 MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.

Targeting strategy

Gene targeting strategy for B-hMMP-8 MC38 cells. The exogenous promoter and human MMP-8 coding sequence was inserted to replace part of murine exon 1-3. The insertion disrupts the endogenous murine Mmp8 gene, resulting in a non-functional transcript.

Protein expression analysis

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MMP-8 expression analysis in B-hMMP-8 MC38 cells by western blot. Human MMP-8 was detected in the supernatant of B-hMMP-8 MC38 cells but not wild-type MC38 cells. The 1-D01 and 1-G12 clones of B-hMMP-8 MC38 cells was used for in vivo experiments.

Tumor growth curve & Body weight changes

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Subcutaneous homograft tumor growth of B-hMMP-8 MC38 cells. B-hMMP-8 MC38 cells (5x106) and wild-type MC38 cells (5x105) were subcutaneously implanted into C57BL/6 mice (female, 12-13-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hMMP-8 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.