B-hPD-L1 plus/hCLDN18.2 MC38_Biocytogen Pharmaceuticals (Beijing) Co., Ltd._Biocytogen, drug development, antibody discovery

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B-hPD-L1 plus/hCLDN18.2 MC38

Common name	B-hPD-L1 plus/hCLDN18.2 MC38	Catalog number	322284
Aliases	CD274;CLDN18	Disease	Colon carcinoma
Organism	Mouse	Strain	C57BL/6
Tissue types	Colon	Tissue	Colon

Description

The exogenous promoter and human PD-L1 CDS was inserted into the mouse Pd-l1 exon 3, the exogenous promoter and human CLDN18.2 CDS was inserted into mouse Rosa26 locus site in B-hPD-L1 plus/hCLDN18.2 MC38. Human PD-L1 and CLDN18.2 were detected on the surface of B-hPD-L1 plus/hCLDN18.2 MC38 cells.

Application

B-hPD-L1 plus/hCLDN18.2 MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.

Targeting strategy

Gene targeting strategy for B-hPD-L1 plus/hCLDN18.2 MC38. The exogenous promoter and human PD-L1 CDS was inserted into the mouse Pd-l1 exon 3, the exogenous promoter and human CLDN18.2 CDS was inserted into mouse Rosa26 locus site in B-hPD-L1 plus/hCLDN18.2 MC38.

Protein expression analysis

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PD-L1 and CLDN18.2 expression analysis in B-hPD-L1 plus/hCLDN18.2 MC38 cells by flow cytometry. Single cell suspensions from B-hPD-L1 plus/hCLDN18.2 MC38 cultures were stained with anti-PD-L1 and anti-CLDN18.2 antibodies. Human PD-L1 and CLDN18.2 were detected on the surface of B-hPD-L1 plus/hCLDN18.2 MC38 cells.

Tumor growth curve & Body weight changes

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Subcutaneous homograft tumor growth of B-hPD-L1 plus/hCLDN18.2 MC38 cells. B-hPD-L1 plus/hCLDN18.2 MC38 cells and wild-type MC38 cells were subcutaneously implanted into C57BL/6N mice (female, 10-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B-hPD-L1 plus/hCLDN18.2 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.

Tumor growth curve of individual mice

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B-hPD-L1 plus/hCLDN18.2 MC38 tumor growth of individual mice. B-hPD-L1 plus/hCLDN18.2 MC38 cells and wild-type MC38 cells were subcutaneously implanted into C57BL/6N mice (female, 10-week-old, n=6). As shown in panel, B-hPD-L1 plus/hCLDN18.2 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.