B-mSiglec15 MC38_Biocytogen Pharmaceuticals (Beijing) Co., Ltd._Biocytogen, drug development, antibody discovery

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B-mSiglec15 MC38

Common name	B-mSiglec15 MC38	Catalog number	311529
Aliases	Cd33l, Cd33l3, EG620235, SIGLEC-I	Disease	Colon carcinoma
Organism	Mouse	Strain	C57BL/6
Tissue types	Colon	Tissue	Colon

Description

The mouse Siglec15 gene was insert into mouse ROSA26 site. Mouse Siglec15 is highly expressed on the surface of B-mSiglec15 MC38 cells.

Application

B-mSiglec15 MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.

Targeting strategy

Gene targeting strategy for B-mSiglec15 MC38.The mouse Siglec15 CDS was insert into mouse ROSA26 site in B-mSiglec15 MC38.

Protein expression analysis

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The Siglec15 expression analysis in B-mSiglec15 MC38 cells by flow cytometry. Single cells were collected from wild-type MC38 and B-mSiglec15 MC38 cells, and analyzed by flow cytometry with anti-human and mouse SIGLEC15 antibody(SIGLEC15-5G12, in house). Mouse Siglec15 was exclusively detectable in B-mSiglec15 MC38 cells but not wild-type MC38 cells. The 2-C12 clone of B-mSiglec15 MC38 cells was used for in vivo experiments.

Tumor growth curve & Body weight changes

Subcutaneous homograft tumor growth of B-mSiglec15 MC38 cells. B-mSiglec15 MC38 cells (5x10⁵) and wild-type MC38 cells (5x10⁵) were subcutaneously implanted into the C57BL/6N mice (female, 5-8 week-old, n=5).Tumor size and mice body weight were measured twice a week. (A) Tumor average volume ± SEM, (B) Mice body weight (Mean± SEM). Volume was expressed in mm³using the formula: V=0.5a X b², where a and b were the long and short diameters of the tumor, respectively. As shown in panel A, B-mSiglec15 MC38 were able to establish tumor in vivo and can be used for efficacy studies.

Tumor growth curve of individual mice

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B-mSiglec15 MC38 tumor cells growth of individual mice. B-mSiglec15 MC38 cells (5x10⁵) and wild-type MC38 cells (5x10⁵ ) were subcutaneously implanted into the C57BL/6N mice (female, 5-8 week-old, n=5). As shown in panel, B-mSiglec15 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.

Protein expression analysis of tumor cells

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B-mSiglec15 MC38 cells were transplanted into C57BL/6N mice (n=5), and on 28 days post inoculation, certain volumes of tumors as was taken to detect the expression level of the cells. Cells were analyzed by flow cytometry with anti-human and mouse SIGLEC15 antibody(SIGLEC15-5G12, in house). As shown, mouse Siglec15 was highly expressed on the surface of B-mSiglec15 MC38 tumor cells. B-mSiglec15 MC38 can be used for in vivo efficacy validation.