top
Please input keywords
Order
*Country
한국
미국
일본
중국
영국
호주
프랑스
독일
이탈리아
싱가포르
인도
러시아
캐나다
스위스
기타 국가
*Province
*City
*Name
*Telephone
*Company
*Position
*Email
*Verification code
*Verification Code
B-hGPR35 mice
Strain Name
 C57BL/6N-Gpr35tm1(GPR35)Bcgen/Bcgen 
Common Name  B-hCCR3 mice
Background C57BL/6N Catalog number 111171
Aliases 
NA
mRNA and Protein expression analysis

from clipboard


Strain specific analysis of GPR35 gene expression in wild-type mice and B-hGPR35 mice by RT-PCR. Mouse Gpr35 mRNA was detectable in splenocytes of wild-type mice (+/+). Human GPR35 mRNA was detectable only in homozygous B-hGPR35 but not in wild-type mice.

Strain specific GPR35 expression analysis in homozygous B-hGPR35 mice by western blot. Stomach, liver and spleen were collected from wild-type mice (+/+) and homozygous B-hGPR35 mice (H/H), and analyzed by western blot with anti-GPR35 antibody. GPR35 was detectable in wild type mice and homozygous B-hGPR35 mice and MCF-7/RAW264.7 cell line, as the antibody is crossly reactive with GPR35 in human and mice.