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B-hLIGHT mice
Strain Name

C57BL/6N-Tnfsf14tm1(TNFSF14)Bcgen/Bcgen

Common Name  B-hLIGHT mice
Background C57BL/6N Catalog number 110944
Aliases 
TNFSF14 (CD258, HVEML, LIGHT, LTg)

Protein expression analysis

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Strain specific LIGHT expression analysis in homozygous B-hLIGHT mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hLIGHT mice (H/H) stimulated with anti-mCD3ε, anti-mCD28 and mouse IL-2 in vitro for 24h, and analyzed by flow cytometry with species-specific anti-LIGHT antibody. Human LIGHT was exclusively detectable on CD8+T cells in homozygous B-hLIGHT but not in wild-type mice.

mRNA expression analysis


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Strain specific analysis of LIGHT gene expression in wild-type C57BL/6 mice and B-hLIGHT mice by RT-PCR. Mouse Light mRNA was detectable only in splenocytes of wild-type C57BL/6 mice (+/+). Human LIGHT mRNA was detectable only in homozygous B-hLIGHT mice (H/H), but not in wild-type mice.