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B-hTREM2 mice(C)
Strain Name
BALB/cCrSlcNifdc-Trem2tm1(TREM2)Bcgen/Bcgen
Common Name  B-hTREM2 mice(C)
Background BALB/cCrSlcNifdc Catalog number 111884
Related Genes 
TREM2, PLOSL2, TREM-2, Trem2a, Trem2b, Trem2c



mRNA expression analysis

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Strain specific analysis of TREM2 gene expression in wild-type mice and B-hTREM2 mice(C) by RT-PCR. Mouse Trem2 mRNA was detectable in wild-type mice (+/+). Human TREM2 mRNA was detectable only in homozygous B-hTREM2 mice(C) (H/H) but not in wild-type mice.

Protein expression analysis

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Strain specific TREM2 expression analysis in homozygous B-hTREM2 mice(C) by flow cytometry. Microglia were collected from wild-type mice (+/+) and homozygous B-hTREM2 mice(C) (H/H), and analyzed by flow cytometry with anti-TREM2 antibody. TREM2 was detectable in microglia of wild-type mice and homozygous B-hTREM2 mice(C), as the antibody is crossly reactive with TREM2 in human and mice. 

In vivo efficacy of anti-human TREM2 antibody

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Antitumor activity of anti-human TREM2 antibody in B-hTREM2 mice(C). (A) Anti-human TREM2 antibody inhibited EMT-6 tumor growth in B-hTREM2 mice(C). Murine breast carcinoma EMT-6 cells were subcutaneously implanted into homozygous B-hTREM2 mice(C) (female, 10 week-old, n=6). Mice were grouped when tumor volume reached approximately 50-80 mm3, and treated with anti-hTREM2 antibody at doses and schedules in panel A. (B) Body weight changes during treatment. As shown in panel A, anti-human TREM2 antibody (in house) were efficacious in controlling tumor growth in B-hTREM2 mice(C), demonstrating they provide a powerful preclinical model for in vivo evaluation of anti-human TREM2 antibody. Values are expressed as mean ± SEM.