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B-hCD300A mice
Strain Name
C57BL/6N-Cd300atm1(CD300A)Bcgen/Bcgen
Common Name  B-hCD300A mice
Background C57BL/6N Catalog number 111428
Aliases 

CD300A:CLM-8, CMRF-35-H9, CMRF-35H, CMRF35-H, CMRF35-H9, 

CMRF35H, CMRF35H9, IGSF12, IRC1, IRC1/IRC2, IRC2, IRp60

mRNA expression analysis

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Strain specific analysis of CD300A gene expression in wild type C57BL/6 mice and homozygous B-hCD300A mice by RT-PCR. Splenocytes were collected from wild type C57BL/6 mice (+/+) and homozygous B-hCD300A mice (H/H). Mouse CD300a mRNA was detectable only in wild type C57BL/6 mice. Human CD300A mRNA was detectable only in homozygous B-hCD300A mice, but not in wild type C57BL/6 mice. 

Protein expression analysis

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Strain specific CD300A expression analysis in homozygous B-hCD300A mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCD300A mice (H/H), and analyzed by flow cytometry with anti-hCD300A antibody. Human CD300A was detectable in B cells, NK cells and DCs  in homozygous B-hCD300A mice (H/H). 

Protein expression analysis

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Strain specific CD300A expression analysis in homozygous B-hCD300A mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCD300A mice (H/H), and analyzed by flow cytometry with anti-hCD300A antibody. Human CD300A was detectable in B cells, NK cells, DCs, neutrophils, monocytes and macrophages in homozygous B-hCD300A mice (H/H).