Western blot analysis of CLEC1A protein expression in homozygous B-hCLEC1A mice. Various tissue lysates were collected from wild-type C57BL/6 mice (+/+)  and homozygous B-hCLEC1A mice (H/H), and then analyzed by western blot with anti-CLEC1A antibody. 40 μg total proteins were loaded for western blotting analysis. hCLEC1A was detected in heart, lung, kidney, small intestine, thymus and lymph node (red arrow). 

Strain specific analysis of CLEC1A mRNA expression in wild-type C57BL/6 mice and B-hCLEC1A mice by RT-PCR. Lung RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hCLEC1A mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human CLEC1A primers. Mouse Clec1a mRNA were detectable only in wild-type C57BL/6 mice. Human CLEC1A mRNA was detectable only in homozygous B-hCLEC1A mice but not in wild-type mice.