| Strain Name |
C57BL/6-Egfrtm2(EGFR)Bcgen/Bcgen
|
Common Name | B-hEGFR mice |
| Background | C57BL/6 | Catalog number | 120771 |
| Aliases |
EGFR, ERBB, ERBB1, ERRP, HER1, NISBD2, PIG61, mENA, epidermal growth factor receptor |
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Strain specific analysis of EGFR gene expression in wild type (WT) mice and B-hEGFR mice by RT-PCR. Mouse Egfr mRNA was detectable only in liver of WT mice (+/+). Human EGFR mRNA was detectable only in homozygous B-hEGFR mice (H/H) but not in WT mice (+/+).
Immunohistochemical (IHC) analysis of EGFR expression in homozygous B-hEGFR mice. The liver and kidney were collected from WT and homozygous B-hEGFR mice (H/H) and analyzed by IHC with anti-EGFR antibody. Mouse EGFR was detectable in WT mice, and human EGFR was detectable in homozygous B-hEGFR mice. The arrow indicates tissue cells with positive EGFR staining (brown).
Complete blood count (CBC).
Serum from the C57BL/6 and B-hEGFR mice (female, 9 week-old，n=8) was collected and analyzed for levels of ALT and AST. There was no differences on either measurement between C57BL/6 and B-hEGFR mice, indicating that introduction of hEGFR in place of its mouse counterpart does not change ALT and AST levels or health of liver. Values are expressed as mean ± SEM.
