Western blot analysis of EPHB3 protein expression in homozygous B-hEPHB3 mice. Various tissue lysates were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hEPHB3 mice (H/H), and then analyzed by proteinsimple-western blot with anti-EPHB3 antibody. 3μg total proteins were loaded for western blotting analysis. The EPHB3  protein was detected in the lung, liver, stomach, brain of wild-type C57BL/6 mice and homozygous B-hEPHB3 mice, as confirmed by cross-reactivity of the antibody against mouse and human EPHB3 proteins.

Strain specific analysis of EPHB3 mRNA expression in wild-type C57BL/6 mice and B-hEPHB3 mice by RT-PCR. Stomach RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hEPHB3 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human EPHB3 primers. Mouse Ephb3 mRNA was detectable only in wild-type C57BL/6 mice. Human EPHB3 mRNA was detectable only in homozygous B-hEPHB3 mice but not in wild-type mice.