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B-hHER2/hTROP2 mice
Strain Name
C57BL/6-Erbb2tm1(ERBB2)Bcgen Tacstd2tm1(TACSTD2)Bcgen/Bcgen 
Common Name  B-hHER2/hTROP2 mice
Background C57BL/6 Catalog number 111881
Related Genes 

TTACSTD2, EGP-1, EGP1, GA733-1, GA7331, GP50, M1S1, TROP2, tumor associated calcium signal transducer 2, 

Trophoblast cell-surface antigen 2 ; ERBB2, CD340, HER-2, HER-2/neu, HER2, MLN 19, NEU, NGL, TKR1, VSCN2

mRNA expression analysis

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Strain specific analysis of HER2 and TROP2 gene expression in wild type (WT) mice and B-hHER2/hTROP2 mice by RT-PCR. Mouse Erbb2 and Tacstd2 mRNA were detectable only in kidney of WT mice (+/+). Human ERBB2 and TACSTD2 mRNA were detectable only in homozygous B-hHER2/hTROP2 mice (H/H;H/H) but not in WT mice (+/+). 

Protein expression analysis

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Strain specific TROP2 expression analysis in homozygous B-hHER2/hTROP2 mice by western blot. Kidney was collected from wild type (WT) mice (+/+) and homozygous B-hHER2/hTROP2 mice (H/H;H/H), and analyzed by western blot with anti-TROP2 antibody. TROP2 was detectable in WT mice (+/+) and homozygous B-hHER2/hTROP2 mice (H/H;H/H) due to the cross-reactivity of antibodies.

IHC analysis of HER2 expression

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Immunohistochemical (IHC) analysis of HER2 expression in homozygous B-hHER2/hTROP2 mice. The liver was collected from WT mice and homozygous B-hHER2/hTROP2 mice and analyzed by IHC with anti-HER2 antibody. HER2 was detectable in WT mice and homozygous B-hHER2/hTROP2 mice due to the cross-reactivity of the antibody. The arrow indicates tissue cells with positive HER2 staining (brown).