Western blot analysis of ORAI1 protein expression in homozygous B-hORAI1 mice. Various tissue lysates were collected from C57BL/6 wildtype mice (+/+) and homozygous B-hORAI1 mice (H/H) , and then analyzed by western blot with species-specific anti-ORAI1 antibody. 32 μg total proteins were loaded for western blotting analysis. Human ORAI1 was detected in spleen/lung/kidney/liver and small intestine of wildtype mice (+/+) and homozygous B-hORAI1 mice (H/H), as the antibody is partly crossly reactive with human and mice.

Strain specific analysis of ORAI1 mRNA expression in wild-type C57BL/6 mice and B-hORAI1 mice by RT-PCR. Spleen and lung RNA were isolated from wildtype C57BL/6 mice (+/+) and homozygous B-hORAI1 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human ORAI1 primers. Mouse Orai1 mRNA was detectable only in wild-type C57BL/6 mice. Human ORAI1 mRNA was detectable only in homozygous B-hORAI1 mice but not in wild-type mice.