B-hTLR4 mice_Biocytogen Pharmaceuticals (Beijing) Co., Ltd._Biocytogen, drug development, antibody discovery

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동물/세포 모델

동물/세포모델

Humanized Mouse Models

Other Humanized Mice

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B-hTLR4 mice

Strain Name	C57BL/6N-Tlr4^{tm1(TLR4)Bcgen}/Bcgen	Common Name	B-hTLR4 mice
Background	C57BL/6N	Catalog number	111893
Aliases	TOLL, CD284, TLR-4, ARMD10

mRNA expression analysis

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Strain specific analysis of TLR4 mRNA expression in wild-type C57BL/6 mice and B-hTLR4 mice by RT-PCR. Spleen RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hTLR4 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human TLR4 primers. Mouse Tlr4 mRNA was detectable only in wild-type C57BL/6 mice. Human TLR4 mRNA was detectable only in homozygous B-hTLR4 mice but not in wild-type mice.

Protein expression analysis

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Strain specific TLR4 expression analysis in wild-type and homozygous B-hTLR4 mice by flow cytometry. Peritoneal cells were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hTLR4 mice (H/H). Flow cytometry analysis of the peritoneal macrophages were detected with anti-TLR4 antibody. Mouse TLR4 was detected on peritoneal macrophages in wild-type mice. Human TLR4 was detected on peritoneal macrophages in homozygous B-hTLR4 mice.