Strain specific analysis of ZG16B mRNA expression in wild-type C57BL/6 mice and B-hZG16B mice by RT-PCR. Parotid gland RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hZG16B mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with human ZG16B primers. Human ZG16B mRNA was detectable only in homozygous B-hZG16B mice but not in wild-type mice. 

Western blot analysis of ZG16B protein expression in homozygous B-hZG16B mice. Various tissue lysates were collected from wild-type C57BL/6 mice and homozygous B-hZG16B mice (H/H), and then analyzed by western blot with anti-human ZG16B antibody. 40 μg total proteins were loaded for western blotting analysis. Human ZG16B was detected in prostate of homozygous B-hZG16B mice.