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B-luc K-562
Common name

 B-luc K-562

Catalog number   310673
Aliases 


NA


Disease Chronic myelogenous leukemia (CML)

Organism

Human

Strain NA
Tissue types

Bone marrow

Tissue Bone marrow

Description 


This B-luc K-562 cell line expresses firefly luciferase as a marker of K-562 cells. Luminescence can be observed B-luc K-562 cells.

Application



B-luc K-562 cell line can be used to evaluate anti-cancer drugs in CML in vivo.


Targeting strategy



Gene targeting strategy for B-luc K-562. The luciferase sequence was inserted in the hAAVS1 locus of wild-type K-562.


In vitro luciferase activity of B-luc K-562 cells 

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Luminescence signal intensity of B-luc K-562 cells. Luminescence intensity was measured using the Bright-GloTM luciferase Assay (Promega, Cat E2610). B-luc K-562 cells have a strong luminescence signal. The mix clone and 1-G08 clone of B-luc K-562 cells can be used in vivo experiments.

Quantitative imaging data & body weight changes 


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Tumor growth and in vivo imaging of B-luc K-562 cells. B-luc K-562 cells (mix, 5x106) were  injected by tail vein into B-NDG mice .Signal intensity and body weight were measured twice a week. (A) Imaging was performed on days 0, 4, 7, 11, 21, and 25, (B) Mice body weight (Mean ± SEM). B-luc K-562 cells can be used for in vivo efficacy evaluation.


Imaging analysis in vivo 

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Quantitative imaging data & body weight changes


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Tumor growth and in vivo imaging of B-luc K-562 cells. B-luc K-562 cells (5x106) were injected into the tail vein of wild-type B-NDG mice. Signal intensity and body weight were measured twice a week. (A) Imaging was performed twice a week. (B) Body weight (Mean ± SEM). B-luc K-562 cells can be used for in vivo efficacy evaluation.

 Imaging analysis in vivo 

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